PMID:2841129
| Citation |
Flachmann, R, Kunz, N, Seifert, J, Gütlich, M, Wientjes, FJ, Läufer, A and Gassen, HG (1988) Molecular biology of pyridine nucleotide biosynthesis in Escherichia coli. Cloning and characterization of quinolinate synthesis genes nadA and nadB. Eur. J. Biochem. 175:221-8 |
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| Abstract |
The two genes, nadA and nadB, responsible for quinolinate biosynthesis from aspartate and dihydroxyacetone phosphate in Escherichia coli were cloned and characterized. Quinolinate (pyridine-2,3-dicarboxylate) is the biosynthetic precursor of the pyridine ring of NAD. Gene nadA was identified by complementation in three different nadA mutant strains. Sequence analysis provided an 840-bp open reading frame coding for a 31,555-Da protein. Gene nadB was identified by complementation in a nadB mutant strain and by the L-aspartate oxidase activity of its gene product. Sequence analysis showed a 1620-bp open reading frame coding for a 60,306-Da protein. For both genes, promoter regions and ribosomal binding sites were assigned by comparison to consensus sequences. The nadB gene product, L-aspartate oxidase, was purified to homogeneity and the N-terminal sequence of 19 amino acids was determined. The enzyme was shown to be specific for L-aspartate. High-copy-number vectors, carrying either gene nadA, nadB or nadA + nadB, increased quinolinate production 1.5-fold, 2.0-fold and 15-fold respectively. Both gene products seem to be equally rate-limiting in quinolinate synthesis. |
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| Keywords |
Amino Acid Oxidoreductases/genetics; Amino Acid Oxidoreductases/isolation & purification; Amino Acid Oxidoreductases/metabolism; Amino Acid Sequence; Bacterial Proteins/genetics; Base Sequence; Cloning, Molecular; DNA Restriction Enzymes; Escherichia coli/genetics; Escherichia coli/metabolism; Genes; Genes, Bacterial; Molecular Sequence Data; NAD/biosynthesis; Plasmids; Promoter Regions, Genetic; Pyridines/metabolism; Quinolinic Acid; Quinolinic Acids/metabolism |
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