PMID:2840553
| Citation |
Said, B, Ghosn, CR, Vu, L and Nunn, WD (1988) Nucleotide sequencing and expression of the fadL gene involved in long-chain fatty acid transport in Escherichia coli. Mol. Microbiol. 2:363-70 |
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| Abstract |
The fadL gene of Escherichia coli codes for an outer membrane protein involved in long-chain fatty acid transport. Its product was purified from outer membrane proteins. We determined the nucleotide sequence of a 2.8-kb chromosomal DNA segment that contains the fadL gene. The fadL gene consists of a 1149-nucleotide coding region and contains a highly hydrophobic polypeptide of 383 amino acids with a calculated molecular weight of 42,380. We have used S1-mapping analysis to identify the transcription initiation site. A region exhibiting extensive dyad symmetry and perfect homology to the catabolite activator protein binding site was detected. |
| Links | |
| Keywords |
Amino Acid Sequence; Bacterial Outer Membrane Proteins/genetics; Bacterial Outer Membrane Proteins/isolation & purification; Base Sequence; Biological Transport; Cyclic AMP/metabolism; DNA Nucleotidyltransferases/genetics; DNA Nucleotidyltransferases/isolation & purification; DNA, Bacterial; Electrophoresis, Polyacrylamide Gel; Escherichia coli/genetics; Escherichia coli Proteins; Fatty Acid Transport Proteins; Fatty Acids/metabolism; Molecular Sequence Data; Plasmids; Receptors, Cyclic AMP/metabolism |
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