PMID:2839184

Citation	Burns, DM and Beacham, IR (1987) Altered localisation of the precursor of a secreted protein in E. coli by a carboxyl-deletion. Biochem. Int. 14:1073-7
Abstract	The effect on protein localisation of a carboxy-terminal deletion of periplasmic UDP-glucose hydrolase (5'-nucleotidase) has been determined in vivo using immunoprecipitation. The processed form of the truncated protein was found in the periplasm; however, in contrast to the situation with the normal protein, the preprotein form of the truncated protein was also found in the periplasm. This result confirms previous semi-in vitro data and supports the suggestion that a conformational change in a membrane-bound intermediate is a normal part of the secretory pathway.
Links	PubMed
Keywords	5'-Nucleotidase; Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Escherichia coli/enzymology; Escherichia coli/genetics; Escherichia coli/metabolism; Genes; Genetic Code; Nucleotidases/genetics; Plasmids; Protein Conformation; Protein Precursors/genetics; Protein Precursors/metabolism
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