PMID:2838786
| Citation |
March, PE, Lerner, CG, Ahnn, J, Cui, X and Inouye, M (1988) The Escherichia coli Ras-like protein (Era) has GTPase activity and is essential for cell growth. Oncogene 2:539-44 |
|---|---|
| Abstract |
The era gene of Escherichia coli encodes a protein (Era) which is similar to the eucaryotic RAS family of proteins. We report here that purified Era possesses both GTP-binding and GTPase activities. Era is also shown to be loosely associated with the inner membrane of E. coli. Overproduction of Era to 5% of the total cellular protein does not apparently alter either cell growth or cAMP levels. Disruption of the era gene by insertional inactivation is shown to be lethal by construction of a conditional lethal era mutant strain. |
| Links | |
| Keywords |
Bacterial Proteins/genetics; Bacterial Proteins/physiology; Cell Division; Cell Membrane/physiology; Cloning, Molecular; DNA Mutational Analysis; Escherichia coli/physiology; GTP Phosphohydrolases/physiology; GTP-Binding Proteins/physiology; Genes, Bacterial; Genes, Lethal; Membrane Proteins/physiology; Phosphoric Monoester Hydrolases/physiology |
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