PMID:2836365
| Citation |
Brill, JA, Quinlan-Walshe, C and Gottesman, S (1988) Fine-structure mapping and identification of two regulators of capsule synthesis in Escherichia coli K-12. J. Bacteriol. 170:2599-611 |
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| Abstract |
Positive and negative regulatory elements involved in the synthesis of colanic acid, the capsular polysaccharide of Escherichia coli K-12, have been identified previously. RcsB, a positive regulator for transcription of the structural genes of colanic acid synthesis (cps), is a protein of about 26 kilodaltons which probably acts as a multimer, rcsC, which maps close to rcsB at 48 min on the E. coli chromosome, exerts a negative effect on expression of the structural genes and codes for a protein of about 100 kilodaltons. The two genes appear to be transcribed in opposite directions, with the C-terminal ends of the genes being less than 0.3 kilobases apart. Multicopy expression of rcsB is lethal in rcsC mutants which carry cps-lac fusions, probably owing to accumulation of intermediates in the capsule synthesis pathway in these cells. Examination of double mutants and cells carrying multicopy rcsB+ plasmids reveal an rcsA-independent pathway for capsule synthesis. We hypothesize that RcsC may act as an environmental sensor, transmitting information to the RcsB positive regulator. |
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| Keywords |
Bacterial Proteins/genetics; Bacteriophage lambda/genetics; Chromosome Mapping; Cloning, Molecular; DNA Transposable Elements; Escherichia coli/genetics; Genes, Regulator; Mutation; Plasmids; Polysaccharides/biosynthesis; Polysaccharides/genetics |
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