PMID:2824448
| Citation |
Vallari, DS and Rock, CO (1987) Isolation and characterization of temperature-sensitive pantothenate kinase (coaA) mutants of Escherichia coli. J. Bacteriol. 169:5795-800 |
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| Abstract |
Escherichia coli mutants conditionally defective in the conversion of pantothenate to coenzyme A were isolated and characterized. The gene was designated coaA and localized between argEH and rpoB near min 90 of the chromosome. The coaA15(Ts) mutation caused a temperature-sensitive growth phenotype and temperature-dependent inactivation of pantothenate kinase activity assayed both in vivo and in vitro. At 30 degrees C, coaA15(Ts) extracts contained less than 20% of the wild-type pantothenate kinase activity; the kinase had near normal kinetic constants for the substrates ATP and pantothenate and was inhibited by coenzyme A to the same degree as the wild-type enzyme. These data define the coaA gene as the structural gene for pantothenate kinase. |
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| Keywords |
Coenzyme A/metabolism; Escherichia coli/enzymology; Escherichia coli/genetics; Genes; Genes, Bacterial; Mutation; Pantothenic Acid/metabolism; Phenotype; Phosphorylation; Phosphotransferases/genetics; Phosphotransferases (Alcohol Group Acceptor); Temperature; Transduction, Genetic; beta-Alanine/metabolism |
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