PMID:2824439
| Citation |
Wanner, BL and Chang, BD (1987) The phoBR operon in Escherichia coli K-12. J. Bacteriol. 169:5569-74 |
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| Abstract |
The phoB and phoR genes encode a transcription activator and a sensory protein of the phosphate regulon, respectively. It is shown here that they were transcribed as an operon in which the phoB gene was promoter proximal. Although an operon structure was suggested previously (K. Makino, H. Shinagawa, M. Amemura, and A. Nakata, J. Mol. Biol. 190:37-44 and 192:549-556, 1986), previous results showed only that phoR gene expression during phosphate limitation is dependent on the upstream phoB promoter. The phoR gene could still have had its own promoter for expression in the presence of phosphate. Two polar transposon-induced mutations are described which simultaneously abolished phoB and phoR gene function in cis; one mutation mapped in the phoB gene, and the other mapped upstream of the phoB gene. These results demonstrate an operon structure, in which phoR gene function required expression from the phoB promoter. Unexpectedly, an antisense pho omega Mu d1(lacZ) insertion within the promoter-proximal end of the phoB gene expressed the lacZ reporter gene, thus allowing for the possibility that the phoBR operon is regulated by an antisense RNA. |
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| Keywords |
Bacterial Proteins/genetics; Bacteriophages/genetics; DNA Transposable Elements; Escherichia coli/genetics; Gene Expression Regulation; Genes, Bacterial; Genetic Complementation Test; Mutation; Operon; Phenotype; Phosphates/metabolism; Promoter Regions, Genetic; Transcription Factors/genetics; Transcription, Genetic |
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