PMID:272656
| Citation |
Shore, D, Dehò, G, Tsipis, J and Goldstein, R (1978) Determination of capsid size by satellite bacteriophage P4. Proc. Natl. Acad. Sci. U.S.A. 75:400-4 |
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| Abstract |
Satellite bacteriophage P4 requires all morphogenic gene products provided by a helper phage, such as coliphage P2, to assemble its own capsid, which is one-third the volume of the larger helper capsid. We have isolated a satellite phage P4 sid (size determination) mutant that is unable to direct the assembly of the small wild-type-size P4 capsid. Instead, this mutant produces P4 plaque-forming units with large P2-size capsids which contain two or three copies of the P4 sid1 genome. P4 sid1 is evidently mutated in a protein that is specifically responsible for determining the precise size and symmetry of the structure into which the helper P2 gene products will assemble. In addition, we have found that the physical size of the genome does not appear to play an essential role in the proper assembly of the icosahedral capsid, since the majority of the P4 sid1 plaque-forming units do not contain a complete capsidful of DNA. |
| Links | |
| Keywords |
Coliphages/genetics; Coliphages/physiology; Coliphages/ultrastructure; DNA, Viral/metabolism; Defective Viruses/physiology; Defective Viruses/ultrastructure; Genes, Viral; Helper Viruses/physiology; Helper Viruses/ultrastructure; Heterozygote; Morphogenesis; Virus Replication |
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