PMID:2689216
| Citation |
Nordlund, P, Uhlin, U, Westergren, C, Joelsen, T, Sjöberg, BM and Eklund, H (1989) New crystal forms of the small subunit of ribonucleotide reductase from Escherichia coli. FEBS Lett. 258:251-4 |
|---|---|
| Abstract |
The small subunit of ribonucleotide reductase from Escherichia coli has been crystallized in two new crystal forms. The form most suitable for X-ray analysis belongs to the orthorhombic space group P2(1)2(1)2(1). It has the cell dimensions 74.3 A, 85.5 A, 115.7 A and diffracts to about 2.1 A resolution. The asymmetric unit most probably contains one dimer. Absorption spectra of single crystals confirm that the crystals contain a binuclear iron center. Crystals of the iron-depleted apoenzyme have also been obtained. |
| Links | |
| Keywords |
Crystallization; Escherichia coli/enzymology; Kinetics; Protein Conformation; Ribonucleotide Reductases/isolation & purification; Ribonucleotide Reductases/metabolism; X-Ray Diffraction |
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