PMID:2684786
| Citation |
Webster, C, Gardner, L and Busby, S (1989) The Escherichia coli melR gene encodes a DNA-binding protein with affinity for specific sequences located in the melibiose-operon regulatory region. Gene 83:207-13 |
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| Abstract |
Crude extracts, made from Escherichia coli cells carrying a plasmid in which the melR gene was expressed from the galP2 promoter, were used as a source of MelR protein. Using DNase I footprinting and gel retardation assays, we show that MelR binds to two sites located from nucleotides (nt) -49 to -75 and -85 to -113, upstream from the melAB transcription start point. The two sites contain identical 18-bp sequences. Specific binding is unaltered by deletions that remove 1 or 6 amino acids (aa) from the C terminus of MelR, but is abolished by deletion of 16, 24 or more aa residues. Sequence homologies between MelR and other DNA-binding proteins are discussed. |
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| Keywords |
Amino Acid Sequence; Base Sequence; Chromosome Deletion; Cloning, Molecular; DNA-Binding Proteins/genetics; Disaccharides; Escherichia coli/genetics; Genes, Bacterial; Genes, Regulator; Melibiose/biosynthesis; Molecular Sequence Data; Nucleotide Mapping; Operon; Plasmids; Promoter Regions, Genetic; Restriction Mapping |
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