PMID:2676977
| Citation |
Ishino, F, Jung, HK, Ikeda, M, Doi, M, Wachi, M and Matsuhashi, M (1989) New mutations fts-36, lts-33, and ftsW clustered in the mra region of the Escherichia coli chromosome induce thermosensitive cell growth and division. J. Bacteriol. 171:5523-30 |
|---|---|
| Abstract |
Three new mutants of Escherichia coli showing thermosensitive cell growth and division were isolated, and the mutations were mapped to the mra region at 2 min on the E. coli chromosome map distal to leuA. Two mutations were mapped closely upstream of ftsI (also called pbpB), in a region of 600 bases; the fts-36 mutant showed thermosensitive growth and formed filamentous cells at 42 degrees C, whereas the lts-33 mutant lysed at 42 degrees C without forming filamentous cells. The mutation in the third new thermosensitive, filament-forming mutant, named ftsW, was mapped between murF and murG. By isolation of these three mutants, about 90% of the 17-kilobase region from fts-36-lts-33 to envA could be filled with genes for cell division and growth, and the genes could be aligned. |
| Links | |
| Keywords |
Base Sequence; Cell Division; DNA Mutational Analysis; Escherichia coli/genetics; Escherichia coli/growth & development; Genes, Bacterial; Genetic Complementation Test; Hot Temperature; Plasmids; Restriction Mapping |
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