PMID:2661831
| Citation |
Hansen, EB (1989) Structure and regulation of the lytic replicon of phage P1. J. Mol. Biol. 207:135-49 |
|---|---|
| Abstract |
Three replicons, R, L and P1dR, have been previously identified in bacteriophage P1, but only the R (or plasmid) replicon has been functionally and structurally characterized. Evidence is provided here that the L-replicon is the principal replicon used for DNA replication during the lytic cycle. The L-replicon (exclusive of its promoter) is shown to be contained within a 1093-base-pair DNA segment that includes a 281-codon open reading frame, designated repL. L-replicon function requires transcription in the direction that should generate translatable repL message. This transcription is normally under the control of the phage c1 repressor, but a deletion that places the functional L-replicon under alternative control can be constructed. The DNA sequence of the replicon and surrounding regions was established. The sequenced region contains the c4 and ant genes and a hitherto unidentified gene, kilA, which is immediately upstream of repL and is controlled by the c1-regulated promoter. The kilA gene was shown to be non-essential for both replication and lytic development whereas the repL gene probably encodes an essential replication protein. |
| Links | |
| Keywords |
Amino Acid Sequence; Base Sequence; Coliphages/genetics; DNA Replication; DNA, Viral/genetics; Escherichia coli; Models, Genetic; Molecular Sequence Data; Mutation; Plasmids; Replicon; Restriction Mapping; Virus Replication |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
See also
References
See Help:References for how to manage references in EcoliWiki.
