PMID:2656640
| Citation |
Horazdovsky, BF and Hogg, RW (1989) Genetic reconstitution of the high-affinity L-arabinose transport system. J. Bacteriol. 171:3053-9 |
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| Abstract |
Expression plasmids containing various portions of araFGH operon sequences were assayed for their ability to facilitate the high-affinity L-arabinose transport process in a strain lacking the chromosomal copy of this operon. Accumulation studies demonstrated that the specific induction of all three operon coding sequences was necessary to restore high-affinity L-arabinose transport. Kinetic analysis of this genetically reconstituted transport system indicated that it functions with essentially wild-type parameters. Therefore, L-arabinose-binding protein-mediated transport appears to require only two inducible membrane-associated components (araG and araH) in addition to the binding protein (araF). |
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| Keywords |
Arabinose/metabolism; Biological Transport, Active; Blotting, Southern; Carrier Proteins/genetics; Cloning, Molecular; DNA Mutational Analysis; DNA, Bacterial/genetics; Escherichia coli/genetics; Escherichia coli/metabolism; Escherichia coli Proteins; Genes, Bacterial; Kinetics; Membrane Proteins/metabolism; Membrane Transport Proteins/genetics; Membrane Transport Proteins/metabolism; Plasmids; Restriction Mapping |
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