PMID:2656633
| Citation |
Masai, H and Arai, K (1989) Escherichia coli dnaT gene function is required for pBR322 plasmid replication but not for R1 plasmid replication. J. Bacteriol. 171:2975-80 |
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| Abstract |
Plasmid pBR322 was unable to replicate in a temperature-sensitive dnaT1 strain at a nonpermissive temperature, whereas a pBR322-derived plasmid carrying the wild-type dnaT+ gene was able to replicate under the same conditions. In contrast to pBR322, plasmid R1 could replicate in the dnaT1 strain at a nonpermissive temperature. In keeping with this finding, in vitro replication of plasmid R1 did not require DnaT protein. |
| Links | |
| Keywords |
DNA Replication; DNA, Bacterial/biosynthesis; DNA, Bacterial/genetics; Escherichia coli/genetics; Genes, Bacterial; Mutation; Plasmids; Regulatory Sequences, Nucleic Acid; Temperature |
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