PMID:2649487
| Citation |
Chu, CC, Templin, A and Clark, AJ (1989) Suppression of a frameshift mutation in the recE gene of Escherichia coli K-12 occurs by gene fusion. J. Bacteriol. 171:2101-9 |
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| Abstract |
The nucleotide sequences of a small gene, racC, and the adjacent N-terminal half of the wild-type recE gene are presented. A frameshift mutation, recE939, inactivating recE and preventing synthesis of the active recE enzyme, exonuclease VIII, was identified. The endpoints of five deletion mutations suppressing recE939 were sequenced. All five delete the frameshift site. Two are intra-recE deletions and fuse the N- and C-terminal portions of recE in frame. Three of the deletions remove the entire N-terminal portion of recE, fusing the C-terminal portion to N-terminal portions of racC in frame. These data indicate that about 70% of the N-terminal half of recE is not required to encode a hypothesized protein domain with exonuclease VIII activity. |
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| Keywords |
Amino Acid Sequence; Base Sequence; Chromosome Deletion; Cloning, Molecular; Escherichia coli/genetics; Escherichia coli Proteins; Exodeoxyribonucleases/genetics; Gene Expression Regulation; Genes, Bacterial; Molecular Sequence Data; Mutation; Recombination, Genetic; Restriction Mapping; Suppression, Genetic |
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