PMID:2646274

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Citation

Scott, D and Amy, NK (1989) Molybdenum accumulation in chlD mutants of Escherichia coli. J. Bacteriol. 171:1284-7

Abstract

The content of molybdenum in wild-type and chlD cells was measured under a variety of growth conditions to determine if cells with a defective chlD gene were able to accumulate molybdenum. The chlD cells accumulated less molybdenum than wild-type cells did but concentrated molybdenum to a level at least 20-fold higher than the concentration in the culture medium. Molybdenum was present within spheroplasts of chlD cells and was not dialyzable. The chlD cells accumulated as much molybdenum as wild-type cells did when grown in medium containing 0.1 mM molybdate; thus, the capability of incorporation of molybdenum into cellular component(s) was equivalent to that of the wild type under these conditions.

Links

PubMed PMC209742

Keywords

Cytochromes/genetics; Escherichia coli/genetics; Escherichia coli/metabolism; Genes; Genes, Bacterial; Molybdenum/metabolism; Mutation; Nitrate Reductases/genetics; Species Specificity; Spheroplasts/metabolism

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