PMID:2644207

Citation	Matsuzawa, H, Asoh, S, Kunai, K, Muraiso, K, Takasuga, A and Ohta, T (1989) Nucleotide sequence of the rodA gene, responsible for the rod shape of Escherichia coli: rodA and the pbpA gene, encoding penicillin-binding protein 2, constitute the rodA operon. J. Bacteriol. 171:558-60
Abstract	The rodA gene, which is responsible for the rod shape of Escherichia coli, was located 5 nucleotides downstream of another rod-shape-determining gene, pbpA, encoding penicillin-binding protein 2. The coding region for the RodA protein was 1,110 base pairs in length. Two plasmids, carrying a rodA-lacZ gene fusion with and without the pbpA promoter upstream of the gene fusion, were constructed. On the basis of the difference between the expression levels of the beta-galactosidase activity dependent on and independent of the pbpA promoter, we concluded that the pbpA and rodA genes constitute a single transcriptional unit called the rodA operon.
Links	PubMed PMC209621
Keywords	Amino Acid Sequence; Bacterial Proteins; Base Sequence; Carrier Proteins/genetics; Cloning, Molecular; Escherichia coli/cytology; Escherichia coli/genetics; Genes; Genes, Bacterial; Hexosyltransferases; Molecular Sequence Data; Muramoylpentapeptide Carboxypeptidase/genetics; Operon; Penicillin-Binding Proteins; Penicillins/metabolism; Peptidyl Transferases; Promoter Regions, Genetic
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