PMID:2574599

Citation	Magasanik, B Regulation of transcription of the glnALG operon of Escherichia coli by protein phosphorylation. Biochimie 71:1005-12
Abstract	The transcription of glnA, the structural gene for glutamine synthetase in enteric bacteria, is regulated by the phosphorylation and dephosphorylation of an effector protein, NRI. In its phosphorylated form the effector activates the initiation of transcription at promoters specific of sigma 54, rather than the abundant sigma 70. The ability of NRI-phosphate to stimulate the formation of open promoter-sigma 54 RNA polymerase complexes is enhanced by specific binding sites, located in the case of glnA 100 and 130 base pairs upstream from the transcriptional start site. These sites can be moved more than 1000 base pairs upstream or downstream without losing their effectiveness. The phosphorylation and dephosphorylation of NRI-NRI-phosphate is catalyzed by the modulator protein NRII. Its activity is controlled by an intracellular signal, the ratio of glutamine to 2-ketoglutarate, which is generated by glutamine synthetase in response to the environmental stimulus, the availability or lack of ammonia. The signal is transduced to the modulator by means of 2 additional proteins: uridylytransferase and PII.
Links	PubMed
Keywords	Bacterial Proteins; DNA-Binding Proteins/genetics; Escherichia coli/genetics; Escherichia coli Proteins; Gene Expression Regulation, Bacterial; Glutamate-Ammonia Ligase/genetics; Operon; PII Nitrogen Regulatory Proteins; Phosphorylation; Trans-Activators; Transcription Factors; Transcription, Genetic
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