PMID:2570347
| Citation |
Kang, WK, Icho, T, Isono, S, Kitakawa, M and Isono, K (1989) Characterization of the gene rimK responsible for the addition of glutamic acid residues to the C-terminus of ribosomal protein S6 in Escherichia coli K12. Mol. Gen. Genet. 217:281-8 |
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| Abstract |
Ribosomal protein S6 of wild-type strains of Escherichia coli contains up to six glutamic acid residues at its C-terminus. The first two residues are encoded by the structural gene for this protein (rpsF) and the rest are added post-translationally. Mutants deficient in this modification were isolated and characterized genetically and biochemically. The S6 protein in these mutants appeared to contain only two glutamic acid residues at the C-terminus as expected. The mutated gene was termed rimK and was mapped at 18.7 min between cmlA and aroA. The rimK gene was cloned into a cosmid vector and its nucleotide sequence determined. Analysis of the transcriptional and translational products of this gene indicates that it encodes a protein with an Mr of 31.5 kDa and that it forms an operon with a gene encoding a 24 kDa protein. An rpsF mutant containing a Glu to Lys replacement in the second residue from the C-terminus of protein S6 was isolated. The S6 protein of this mutant was apparently inaccessible to the RimK modification system. This indicates that the RimK modification system requires the wild-type amino acid sequence at least in the C-terminal region of ribosomal protein S6. |
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| Keywords |
Amino Acid Sequence; Base Sequence; Chromosome Mapping; Cloning, Molecular; DNA, Bacterial/genetics; Escherichia coli/genetics; Escherichia coli/metabolism; Genes, Bacterial; Glutamates/metabolism; Glutamic Acid; Molecular Sequence Data; Mutation; Plasmids; Protein Biosynthesis; Protein Processing, Post-Translational; Ribosomal Protein S6; Ribosomal Proteins/genetics; Ribosomal Proteins/metabolism; Transcription, Genetic |
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