PMID:2558284
| Citation |
Birkenbihl, RP and Vielmetter, W (1989) Complete maps of IS1, IS2, IS3, IS4, IS5, IS30 and IS150 locations in Escherichia coli K12. Mol. Gen. Genet. 220:147-53 |
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| Abstract |
In this paper complete distribution maps are presented of the seven IS elements 1, 2, 3, 4, 5, 30 and 150. These maps were obtained during the construction of an almost complete restriction map of the Escherichia coli genome of K12 strain BHB2600. The positions of IS elements were correlated to this map. The distribution of integration sites of all IS types is nonrandom. Besides a large gap from 79 min to 96 min, there is a pronounced IS cluster at 6 min and another at 97 min, map locations that have low gene incidences on the classical map. One cluster coincides with a region of IS induced rearrangements. The IS distribution pattern was compared to patterns of strains W3110 and HB101. |
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| Keywords |
Cosmids; DNA Transposable Elements; DNA, Bacterial/genetics; Deoxyribonuclease EcoRI; Escherichia coli/genetics; Gene Amplification; Restriction Mapping |
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