PMID:2547660
| Citation |
McEachern, F and Fisher, LM (1989) Regulation of DNA supercoiling in Escherichia coli: genetic basis of a compensatory mutation in DNA gyrase. FEBS Lett. 253:67-70 |
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| Abstract |
Bacterial DNA supercoiling is controlled by balancing the supercoiling activity of DNA gyrase and the relaxing activity of DNA topoisomerase I. We have characterized the gyrB gene from a top A deletion mutant of Escherichia coli (DM800) that has a compensatory mutation in gyrB, lowering the activity of gyrase 10-fold, and thereby redressing the intracellular level of supercoiling. The mutant gene differs from the wild type in carrying three rather than two direct tandem repeats of a 6 bp sequence encoding Ala-Arg. We suggest this novel mutation affects domain spacing and was generated by an unequal crossing over event, possibly involving gyrase. |
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| Keywords |
Base Sequence; DNA Topoisomerases, Type II/genetics; DNA, Superhelical/genetics; Escherichia coli/genetics; Escherichia coli/ultrastructure; Genes, Bacterial; Mutation; Restriction Mapping; Structure-Activity Relationship |
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