PMID:2545537
| Citation |
Nellemann, LJ, Holm, F, Atlung, T and Hansen, FG (1989) Cloning and characterization of the Escherichia coli phosphoglycerate kinase (pgk) gene. Gene 77:185-91 |
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| Abstract |
The pgk gene of Escherichia coli coding for the phosphoglycerate kinase was subcloned from the Carbon and Clarke collection plasmid pLC33-5. The position and direction of transcription of the pgk gene was determined by Tn5 insertion mutagenesis. Analysis of proteins encoded from these plasmids showed that the pgk gene product is a 40-kDa protein, and that the gene is transcribed from two promoters, one immediately in front of the gene and one in front of an upstream gene coding for a 38-kDa polypeptide of unknown function. The position of the Pgk protein on two-dimensional O'Farrel gels was identified, and from this we conclude that it is one of the proteins induced by anaerobiosis [Smith and Neidhardt, J. Bacteriol. 154 (1987) 336-343]. The pgk gene was also found to show growth phase regulation; the synthesis of Pgk protein was induced more than ten-fold during transition from the exponential to the stationary growth phase. |
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| Keywords |
Anaerobiosis; Cell Cycle; Cloning, Molecular; DNA Transposable Elements; Electrophoresis, Polyacrylamide Gel; Escherichia coli/enzymology; Escherichia coli/genetics; Escherichia coli/growth & development; Gene Expression Regulation; Genes, Bacterial; Mutation; Phosphoglycerate Kinase/biosynthesis; Phosphoglycerate Kinase/genetics; Plasmids; Restriction Mapping; Transcription, Genetic |
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