PMID:2542219
| Citation |
Fath, MJ, Mahanty, HK and Kolter, R (1989) Characterization of a purF operon mutation which affects colicin V production. J. Bacteriol. 171:3158-61 |
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| Abstract |
A mini-Tn10-kan insertion mutation identified a gene in the chromosome of Escherichia coli required for colicin V production from plasmid pColV-K30. With the complete restriction map of E. coli, the mutation was rapidly mapped to 50.0 min, within the purF operon. Sequence analysis showed that the insertion occurred in a gene with no previously known function which is located directly upstream of purF. We designated this gene cvpA for colicin V production. The mutant requires adenine for growth, probably because of a polar effect on purF expression. However, an adenine auxotroph showed no defect in colicin V production, suggesting that the cvpA mutation is responsible for the effect on colicin V production. Two possible models of cvpA1 allele function are discussed. |
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| Keywords |
Alleles; Bacteriocin Plasmids; Chromosomes, Bacterial/physiology; Cloning, Molecular; Colicins/biosynthesis; DNA Transposable Elements; DNA, Bacterial/genetics; Escherichia coli/genetics; Gene Expression Regulation; Genes, Bacterial; Operon; Plasmids; Purines/physiology; Restriction Mapping |
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