PMID:2541439
| Citation |
Sak, BD, Eisenstark, A and Touati, D (1989) Exonuclease III and the catalase hydroperoxidase II in Escherichia coli are both regulated by the katF gene product. Proc. Natl. Acad. Sci. U.S.A. 86:3271-5 |
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| Abstract |
The levels of both exonuclease III (exo III, product of xthA) and hydroperoxidase II (HP-II, product of katE) activity in Escherichia coli were influenced by a functional katF gene. The katF gene product is also necessary for synthesis of HP-II. Mutations in either katF or xthA, but not katE, result in sensitivity to H2O2 and near-UV (300-400 nm) radiation. Exo III, encoded by the xthA locus, recognizes and removes nucleoside 5'-monophosphates near apurinic and apyrimidinic sites in damaged DNA. Extracts of katF mutant strains had little detectable exo III activity. When a katF+ plasmid was introduced into the katF mutant, exo III activity exceeded wild-type levels. We propose that the katF gene is a trans-acting positive regulator of exo III and HP-II enzymes, both of which are involved in cellular recovery from oxidative damage. |
| Links | |
| Keywords |
Apurinic Acid/metabolism; DNA Damage; Escherichia coli/drug effects; Escherichia coli/enzymology; Escherichia coli/genetics; Escherichia coli/radiation effects; Exodeoxyribonucleases/genetics; Exodeoxyribonucleases/metabolism; Gene Expression Regulation; Genes, Bacterial; Genes, Regulator; Hydrogen Peroxide/pharmacology; Mutation; Peroxidase/genetics; Peroxidase/metabolism; Plasmids; Polynucleotides/metabolism; Transformation, Bacterial; Ultraviolet Rays |
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