PMID:2537825
| Citation |
Atlung, T, Nielsen, A and Hansen, FG (1989) Isolation, characterization, and nucleotide sequence of appY, a regulatory gene for growth-phase-dependent gene expression in Escherichia coli. J. Bacteriol. 171:1683-91 |
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| Abstract |
A plasmid carrying a regulator gene, designated appY, was found in the screening of an Escherichia coli gene library for clones overproducing AppA, an acid phosphatase which is induced as a culture approaches the stationary phase. In cells containing multicopy plasmids carrying the appY gene, the expression of the chromosomal appY gene was stimulated 10- to 40-fold in the stationary phase and more than 100-fold during exponential growth. The appA plasmid also changed the rate of synthesis of more than 30 other proteins in a growth-phase-dependent way. The appY gene was mapped to 13 min on the E. coli genetic map. The position of the appY gene on the 4.9-kilobase HindIII fragment of the original clone was located by Tn5 mutagenesis and deletion analysis, and the nucleotide sequence of a 1.9-kilobase region containing the gene was determined. The appY gene product was identified as a weakly expressed 243-amino-acid polypeptide which contains a stretch of 20 amino acids showing very good similarity to the conserved DNA-binding domain of repressors and transcriptional activators. |
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| Keywords |
Amino Acid Sequence; Bacterial Proteins/genetics; Base Sequence; Cloning, Molecular; Escherichia coli/genetics; Escherichia coli/growth & development; Gene Expression Regulation; Genes, Bacterial; Genes, Regulator; Genotype; Molecular Sequence Data; Plasmids; Restriction Mapping |
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