PMID:2537822

Citation	Lipinska, B, Fayet, O, Baird, L and Georgopoulos, C (1989) Identification, characterization, and mapping of the Escherichia coli htrA gene, whose product is essential for bacterial growth only at elevated temperatures. J. Bacteriol. 171:1574-84
Abstract	We identified and cloned an Escherichia coli gene called htrA (high temperature requirement). The htrA gene was originally discovered because mini-Tn10 transposon insertions in it allowed E. coli growth at 30 degrees C but prevented growth at elevated temperatures (above 42 degrees C). The htrA insertion mutants underwent a block in macromolecular synthesis and eventually lysed at the nonpermissive temperature. The htrA gene was located at approximately 3.7 min (between the fhuA and dapD loci) on the genetic map of E. coli and between 180 and 187.5 kilobases on the physical map. It coded for an unstable, 51-kilodalton protein which was processed by removal of an amino-terminal fragment, resulting in a stable, 48-kilodalton protein.
Links	PubMed PMC209783
Keywords	Chromosome Mapping; Chromosomes, Bacterial; Cloning, Molecular; DNA Transposable Elements; Escherichia coli/genetics; Escherichia coli/growth & development; Genes, Bacterial; Genotype; Mutation; Phenotype; Plasmids; Protein Biosynthesis; Temperature; Transcription, Genetic; Transduction, Genetic
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