PMID:2536712
| Citation |
Wahle, E, Lasken, RS and Kornberg, A (1989) The dnaB-dnaC replication protein complex of Escherichia coli. I. Formation and properties. J. Biol. Chem. 264:2463-8 |
|---|---|
| Abstract |
The complex formed between the dnaB and dnaC replication proteins of Escherichia coli is stabilized by ATP binding to dnaC. The dnaB6-dnaC6-ATP6 complex can be maintained without ATP hydrolysis at a concentration as low as 5 x 10(-10) M. The complex is also formed with adenosine 5'-(gamma-thio)triphosphate but generates little or no dnaB activity, suggesting a requirement for ATP hydrolysis in the subsequent stage of binding of the complex to DNA. In this step, dnaC is released, leaving dnaB to function on the associated DNA. |
| Links | |
| Keywords |
Adenosine Triphosphate/analogs & derivatives; Adenosine Triphosphate/metabolism; Bacterial Proteins/metabolism; DNA Replication; Escherichia coli/genetics; Escherichia coli/metabolism; Ethylmaleimide/pharmacology; Kinetics; Macromolecular Substances; Molecular Weight |
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