PMID:2536686
| Citation |
Milner, JL and Wood, JM (1989) Insertion proQ220::Tn5 alters regulation of proline porter II, a transporter of proline and glycine betaine in Escherichia coli. J. Bacteriol. 171:947-51 |
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| Abstract |
Mutation pro-220::Tn5, which increases the resistance of Escherichia coli to 3,4-dehydroproline (M. E. Stalmach, S. Grothe, and J. M. Wood, J. Bacteriol. 156:481-486, 1983), is not linked to putP, proP, or proU. It was located at 40.4 min on the E. coli chromosomal linkage map, by conjugational and transductional mapping, and is now denoted proQ220::Tn5. Proline porter II was not detectable when proQ220::Tn5 proP+ bacteria were cultivated under optimal conditions or with nutritional stress (amino acid limitation). Toxic proline analog sensitivity and proline porter II activity were partially restored to proQ220::Tn5 proP+ bacteria, but not to a proQ220::Tn5 proP219 strain, by a hyperosmotic shift and by growth under osmotic stress. Elevated expression of a proP::lacZ gene fusion, for bacteria grown under osmotic stress, was not influenced by the proQ220::Tn5 insertion. We propose that the proQ locus encodes a positive regulatory element which elevates proline porter II activity. |
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| Keywords |
Alleles; Amino Acid Transport Systems, Neutral; Betaine/metabolism; Biological Transport; DNA Transposable Elements; Escherichia coli/genetics; Escherichia coli/metabolism; Gene Expression Regulation; Genes; Genes, Bacterial; Genotype; Membrane Transport Proteins/genetics; Membrane Transport Proteins/metabolism; Mutation; Phenotype; Proline/metabolism; Transcription, Genetic |
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