PMID:2512996
| Citation |
Cortay, JC, Bleicher, F, Duclos, B, Cenatiempo, Y, Gautier, C, Prato, JL and Cozzone, AJ Utilization of acetate in Escherichia coli: structural organization and differential expression of the ace operon. Biochimie 71:1043-9 |
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| Abstract |
Growth of Escherichia coli on acetate as the sole source of carbon and energy requires operation of the glyoxylate bypass in connection with the expression of the polycistronic ace operon. The structural organization of this operon is presented, including the 3 structural genes coding respectively for malate synthase (aceB), isocitrate lyase (aceA) and isocitrate dehydrogenase kinase/phosphatase (aceK), and the surrounding genes iclR and metA. In addition, the differential expression of genes aceB, aceA, and aceK has been tested both in vivo in a minicell system and in vitro in a plasmid-directed transcription-translation coupled system. Moreover, the codon usage and adaptation to transfer RNA frequencies during translation of the corresponding messenger RNAs have been measured. |
| Links | |
| Keywords |
Acetates/metabolism; Escherichia coli/genetics; Escherichia coli/metabolism; Gene Expression; Genes; Operon; Phosphorylation; RNA, Messenger/genetics |
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