PMID:2504697
| Citation |
Su, HS, Lang, BF and Newman, EB (1989) L-serine degradation in Escherichia coli K-12: cloning and sequencing of the sdaA gene. J. Bacteriol. 171:5095-102 |
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| Abstract |
A new mutant of Escherichia coli K-12 unable to grow with L-serine, glycine, and L-leucine has been isolated by lambda plac Mu insertion and shown to be deficient in L-serine deaminase activity. The corresponding gene, sdaA, has been cloned from a prototrophic strain, and the clone has been characterized and sequenced. The evidence is consistent with the hypothesis that sdaA is the structural gene for L-serine deaminase. However, other possibilities are also considered. No significant homology with previously reported DNA or protein sequences was detected. |
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| Keywords |
Amino Acid Sequence; Base Sequence; Cloning, Molecular; Coliphages/genetics; Escherichia coli/enzymology; Escherichia coli/genetics; Genes; Genes, Bacterial; L-Serine Dehydratase/genetics; Molecular Sequence Data; Plasmids; Restriction Mapping; Serine/metabolism |
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