PMID:2468648
| Citation |
Saporito, SM, Gedenk, M and Cunningham, RP (1989) Role of exonuclease III and endonuclease IV in repair of pyrimidine dimers initiated by bacteriophage T4 pyrimidine dimer-DNA glycosylase. J. Bacteriol. 171:2542-6 |
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| Abstract |
The role of exonuclease III and endonuclease IV in the repair of pyrimidine dimers in bacteriophage T4-infected Escherichia coli was examined. UV-irradiated T4 showed reduced survival when plated on an xth nfo double mutant but showed wild-type survival on either single mutant. T4 denV phage were equally sensitive when plated on wild-type E. coli or an xth nfo double mutant, suggesting that these endonucleases function in the same repair pathway as T4 pyrimidine dimer-DNA glycosylase. A uvrA mutant of E. coli in which the repair of pyrimidine dimers was dependent on the T4 denV gene carried on a plasmid was constructed. Neither an xth nor an nfo derivative of this strain was more sensitive than the parental strain to UV irradiation. We were unable to construct a uvrA xth nfo triple mutant. In addition, T4, which turns off the host UvrABC excision nuclease, showed reduced plating efficiency on an xth nfo double mutant. |
| Links | |
| Keywords |
Apurinic Acid/metabolism; DNA Glycosylases; DNA Repair; DNA-(Apurinic or Apyrimidinic Site) Lyase; Deoxyribonuclease IV (Phage T4-Induced); Endodeoxyribonucleases/physiology; Escherichia coli/genetics; Escherichia coli Proteins; Exodeoxyribonucleases/physiology; Mutation; N-Glycosyl Hydrolases/metabolism; Polynucleotides/metabolism; Pyrimidine Dimers/metabolism; T-Phages/enzymology; T-Phages/genetics; T-Phages/radiation effects; Ultraviolet Rays; Virus Replication |
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