PMID:2407854
| Citation |
Portier, C, Dondon, L and Grunberg-Manago, M (1990) Translational autocontrol of the Escherichia coli ribosomal protein S15. J. Mol. Biol. 211:407-14 |
|---|---|
| Abstract |
When rpsO, the gene encoding the ribosomal protein S15 in Escherichia coli, is carried by a multicopy plasmid, the mRNA synthesis rate of S15 increases with the gene dosage but the rate of synthesis of S15 does not rise. A translational fusion between S15 and beta-galactosidase was introduced on the chromosome in a delta lac strain and the expression of beta-galactosidase studied under different conditions. The presence of S15 in trans represses the beta-galactosidase level five- to sixfold, while the synthesis rate of the S15-beta-galactosidase mRNA decreases by only 30 to 50%. These data indicate that S15 is subject to autogenous translational control. Derepressed mutants were isolated and sequenced. All the point mutations map in the second codon of S15, suggesting a location for the operator site that is very near to the translation initiation codon. However, the creation of deletion mutations shows that the operator extends into the 5' non-coding part of the message, thus overlapping the ribosome loading site. |
| Links |
PubMed Online version:10.1016/0022-2836(90)90361-O |
| Keywords |
Base Composition; Base Sequence; Escherichia coli/genetics; Genes, Bacterial; Molecular Sequence Data; Nucleic Acid Conformation; Oligonucleotide Probes; Plasmids; Protein Biosynthesis; RNA, Messenger/genetics; Restriction Mapping; Ribosomal Proteins/genetics; Transcription, Genetic |
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