PMID:2407558
| Citation |
Warren, MJ, Stolowich, NJ, Santander, PJ, Roessner, CA, Sowa, BA and Scott, AI (1990) Enzymatic synthesis of dihydrosirohydrochlorin (precorrin-2) and of a novel pyrrocorphin by uroporphyrinogen III methylase. FEBS Lett. 261:76-80 |
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| Abstract |
Uroporphyrinogen III methylase was purified from a recombinant hemB-strain of E. coli harbouring a plasmid containing the cysG gene. N-terminal analysis of this purified protein gave an amino acid sequence corresponding to that predicted from the genetic code. From the u.v./visible spectrum of the reaction catalysed by this SAM dependent methylase it was possible to observe the sequential appearance of the chromophores of a dipyrrocorphin and subsequently of a pyrrocorphin. Confirmation of this transformation was obtained from 13C-NMR studies when it was demonstrated, for the first time directly, that uroporphyrinogen is initially converted into dihydrosirohydrochlorin (precorrin-2) and then, by further methylation, into a novel trimethylpyrrocorphin. |
| Links | |
| Keywords |
Amino Acid Sequence; Chromatography, High Pressure Liquid; Escherichia coli/enzymology; Magnetic Resonance Spectroscopy; Methylation; Methyltransferases/metabolism; Molecular Sequence Data; Molecular Structure; Porphyrins/biosynthesis; Spectrophotometry; Uroporphyrins/biosynthesis; Vitamin B 12/biosynthesis |
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