PMID:2407239
| Citation |
Hawkes, TR, Lewis, T, Coggins, JR, Mousdale, DM, Lowe, DJ and Thorneley, RN (1990) Chorismate synthase. Pre-steady-state kinetics of phosphate release from 5-enolpyruvylshikimate 3-phosphate. Biochem. J. 265:899-902 |
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| Abstract |
The pre-steady-state kinetics of phosphate formation from 5-enolpyruvylshikimate 3-phosphate catalysed by Escherichia coli chorismate synthase (EC 4.6.1.4) were studied by a rapid-acid-quench technique at 25 degrees C at pH 7.5. No pre-steady-state 'burst' or 'lag' phase was observed, showing that phosphate is released concomitant with the rate-limiting step of the enzyme. The implications of this result for the mechanism of action of chorismate synthase are discussed. |
| Links | |
| Keywords |
Binding Sites; Chemical Phenomena; Chemistry; Escherichia coli/enzymology; Kinetics; Lyases/metabolism; Phosphates/metabolism; Phosphorus-Oxygen Lyases; Shikimic Acid/analogs & derivatives; Shikimic Acid/metabolism |
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