PMID:2404794
| Citation |
Krone, FA, Westphal, G, Meyer, HE and Schwenn, JD (1990) PAPS-reductase of Escherichia coli. Correlating the N-terminal amino acid sequence with the DNA of gene cys H. FEBS Lett. 260:6-9 |
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| Abstract |
The DNA of the gene complementing a PAPS-reductase-deficient strain of Escherichia coli was sequenced. The N-terminal amino acid sequence of the purified PAPS-reductase confirmed that cys H is the structural gene for this enzyme. The open reading frame extends 732 bases and encodes for a peptide of Mr = 27927. The gene product is functionally active when supplemented with thioredoxin and immunologically related with the wild type enzyme. |
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| Keywords |
Amino Acid Sequence; Blotting, Western; DNA/analysis; Escherichia coli/enzymology; Escherichia coli/genetics; Gene Expression Regulation, Enzymologic; Genes, Bacterial; Molecular Sequence Data; Mutation; Oxidoreductases/analysis; Oxidoreductases/genetics; Plasmids; Recombinant Proteins/analysis |
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