PMID:2404765
| Citation |
Meng, LM, Kilstrup, M and Nygaard, P (1990) Autoregulation of PurR repressor synthesis and involvement of purR in the regulation of purB, purC, purL, purMN and guaBA expression in Escherichia coli. Eur. J. Biochem. 187:373-9 |
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| Abstract |
The purR gene encodes a repressor (PurR) controlling the synthesis of the enzymes of purine biosynthesis. The subunit of PurR was identified as a 38-kDa polypeptide by SDS/polyacrylamide gel electrophoresis. Analysis of a purR-lacZ transcriptional fusion indicated that purR expression is autoregulated. This was confirmed by gel retardation and DNaseI footprinting experiments, where two PurR-binding sites were identified in the transcribed part of purR. Introduction of a purR mutation in wild-type and pur-lac fusion strains was found to abolish purine repression of all genes of the purine biosynthetic pathway except for purA. |
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| Keywords |
Base Sequence; Binding Sites; DNA/analysis; Electrophoresis, Polyacrylamide Gel; Escherichia coli/genetics; Escherichia coli/metabolism; Gene Expression Regulation, Bacterial; Genes, Bacterial; Molecular Sequence Data; Mutation; Operator Regions, Genetic; Plasmids; Purines/metabolism; Repressor Proteins/analysis; Repressor Proteins/biosynthesis; Repressor Proteins/physiology; Transcription Factors/biosynthesis |
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