PMID:2403546
| Citation |
Petersen, C (1990) Escherichia coli ribosomal protein L10 is rapidly degraded when synthesized in excess of ribosomal protein L7/L12. J. Bacteriol. 172:431-6 |
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| Abstract |
In Escherichia coli the genes encoding ribosomal proteins L10 and L7/12, rplJ and rplL, respectively, are cotranscribed and subject to translational coupling. Synthesis of both proteins is coordinately regulated at the translational level by binding of L10 or a complex of L10 and L7/L12 to a single target in the mRNA leader region upstream of rplJ. Unexpectedly, small deletions that inactivated the ribosome-binding site of the rplL gene carried on multicopy plasmids exerted a negative effect on expression of the upstream rplJ gene. This effect could be overcome by overproduction of L7/L12 in trans from another plasmid. This apparent stimulation resulted from stabilization of the overproduced L10 protein by L7/L12, presumably because free L10, in contrast to L10 complexed with L7/L12, is subject to rapid proteolytic decay. The contribution of this decay mechanism to the regulation of the rplJL operon is evaluated. |
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| Keywords |
Bacterial Proteins/biosynthesis; Base Sequence; Chromosome Deletion; Escherichia coli/metabolism; Gene Expression Regulation, Bacterial; Genes, Bacterial; Molecular Sequence Data; Operon; Ribosomal Proteins/biosynthesis; Ribosomal Proteins/genetics; Ribosomal Proteins/metabolism |
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