PMID:237907
| Citation |
Harwood, JP and Peterkofsky, A (1975) Glucose-sensitive adenylate cyclase in toluene-treated cells of Escherichia coli B. J. Biol. Chem. 250:4656-62 |
|---|---|
| Abstract |
Toluene treatment of Escherichia coli B makes it possible to measure adenylate cyclase activity directly using [alpha-32-P]-ATP as substrate. In contrast to French press extracts, the activity of adenylate cyclase in toluene-treated cells shows many of the characteristics of the enzyme seen in the intact cell. In both toluene-treated and intact cells the activity of adenylate cyclase is inhibited at least 85% by glucose, while in French press extracts the enzyme activity is much lower and is not sensitive to inhibition by glucose. In toluene-treated cells, glucose inhibits at 10 muM, and the effect is rapid in onset and readily reversible. The activity is not inhibited by glucose 6-phosphate suggesting that glucose is responsible for the inhibition. The measurement of the activity and sensitivity to glucose of adenylate cyclase in toluene-treated cells requires the presence of potassium phosphate in the assay medium. Since it does not increase the activity or sensitivity of the enzyme in the French press extract, it is suggested that potassium phosphate is required for the maintenance of cellular integrity necessary for the activity and sensitivity of adenylate cyclase. |
| Links | |
| Keywords |
Adenylate Cyclase/isolation & purification; Adenylate Cyclase/metabolism; Ammonium Chloride/pharmacology; Chromatography, Ion Exchange; Cyclic AMP/pharmacology; Enzyme Activation/drug effects; Escherichia coli/drug effects; Escherichia coli/enzymology; Glucose/pharmacology; Hydrogen-Ion Concentration; Kinetics; Phosphates/pharmacology; Potassium/pharmacology; Potassium Chloride/pharmacology; Sodium/pharmacology; Sodium Chloride/pharmacology; Sulfates/pharmacology; Temperature; Theophylline/pharmacology; Time Factors; Toluene/pharmacology |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
See also
References
See Help:References for how to manage references in EcoliWiki.