PMID:23755967
| Citation |
McCallin, S, Alam Sarker, S, Barretto, C, Sultana, S, Berger, B, Huq, S, Krause, L, Bibiloni, R, Schmitt, B, Reuteler, G and Brüssow, H (2013) Safety analysis of a Russian phage cocktail: from metagenomic analysis to oral application in healthy human subjects. Virology 443:187-96 |
|---|---|
| Abstract |
Phage therapy has a long tradition in Eastern Europe, where preparations are comprised of complex phage cocktails whose compositions have not been described. We investigated the composition of a phage cocktail from the Russian pharmaceutical company Microgen targeting Escherichia coli/Proteus infections. Electron microscopy identified six phage types, with numerically T7-like phages dominating over T4-like phages. A metagenomic approach using taxonomical classification, reference mapping and de novo assembly identified 18 distinct phage types, including 7 genera of Podoviridae, 2 established and 2 proposed genera of Myoviridae, and 2 genera of Siphoviridae. De novo assembly yielded 7 contigs greater than 30 kb, including a 147-kb Myovirus genome and a 42-kb genome of a potentially new phage. Bioinformatic analysis did not reveal undesired genes and a small human volunteer trial did not associate adverse effects with oral phage exposure. |
| Links |
PubMed Online version:10.1016/j.virol.2013.05.022 |
| Keywords |
Administration, Oral; Bacteriophages/classification; Bacteriophages/genetics; Bacteriophages/ultrastructure; Biological Therapy/adverse effects; Biological Therapy/methods; Escherichia coli Infections/therapy; Gram-Negative Bacteria/classification; Gram-Negative Bacteria/virology; Humans; Metagenomics/methods; Microscopy, Electron, Transmission; Myoviridae/classification; Myoviridae/genetics; Myoviridae/ultrastructure; Podoviridae/classification; Podoviridae/genetics; Podoviridae/ultrastructure; Proteus Infections/therapy; Russia; Siphoviridae/classification; Siphoviridae/genetics; Siphoviridae/ultrastructure; Treatment Outcome |
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