PMID:23401005

Citation	Akopian, D, Dalal, K, Shen, K, Duong, F and Shan, SO (2013) SecYEG activates GTPases to drive the completion of cotranslational protein targeting. J. Cell Biol. 200:397-405
Abstract	Signal recognition particle (SRP) and its receptor (SR) comprise a highly conserved cellular machine that cotranslationally targets proteins to a protein-conducting channel, the bacterial SecYEG or eukaryotic Sec61p complex, at the target membrane. Whether SecYEG is a passive recipient of the translating ribosome or actively regulates this targeting machinery remains unclear. Here we show that SecYEG drives conformational changes in the cargo-loaded SRP-SR targeting complex that activate it for GTP hydrolysis and for handover of the translating ribosome. These results provide the first evidence that SecYEG actively drives the efficient delivery and unloading of translating ribosomes at the target membrane.
Links	PubMed PMC3575545 Online version:10.1083/jcb.201208045
Keywords	Escherichia coli/metabolism; Escherichia coli Proteins/physiology; Guanosine Triphosphate/metabolism; Hydrolysis; Models, Biological; Protein Structure, Tertiary; Protein Transport/physiology; Receptors, Cytoplasmic and Nuclear/chemistry; Receptors, Cytoplasmic and Nuclear/metabolism; Receptors, Cytoplasmic and Nuclear/physiology; Receptors, Peptide/chemistry; Receptors, Peptide/metabolism; Receptors, Peptide/physiology; Ribosomes/metabolism; Ribosomes/physiology; Signal Recognition Particle/chemistry; Signal Recognition Particle/metabolism; Signal Recognition Particle/physiology
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