PMID:23235161
| Citation |
Nurse, P and Marians, KJ (2013) Purification and characterization of Escherichia coli MreB protein. J. Biol. Chem. 288:3469-75 |
|---|---|
| Abstract |
The actin homolog MreB is required in rod-shaped bacteria for maintenance of cell shape and is intimately connected to the holoenzyme that synthesizes the peptidoglycan layer. The protein has been reported variously to exist in helical loops under the cell surface, to rotate, and to move in patches in both directions around the cell surface. Studies of the Escherichia coli protein in vitro have been hampered by its tendency to aggregate. Here we report the purification and characterization of native E. coli MreB. The protein requires ATP hydrolysis for polymerization, forms bundles with a left-hand twist that can be as long as 4 μm, forms sheets in the presence of calcium, and has a critical concentration for polymerization of 1.5 μM. |
| Links |
PubMed PMC3561565 Online version:10.1074/jbc.M112.413708 |
| Keywords |
Adenosine Triphosphate/pharmacology; Escherichia coli/metabolism; Escherichia coli Proteins/isolation & purification; Escherichia coli Proteins/ultrastructure; Magnesium/pharmacology; Polymerization/drug effects |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
See also
References
See Help:References for how to manage references in EcoliWiki.