PMID:23233451
| Citation |
Shimada, T, Katayama, Y, Kawakita, S, Ogasawara, H, Nakano, M, Yamamoto, K and Ishihama, A (2012) A novel regulator RcdA of the csgD gene encoding the master regulator of biofilm formation in Escherichia coli. Microbiologyopen 1:381-94 |
|---|---|
| Abstract |
The FixJ/LuxR family transcription factor CsgD is a master regulator of biofilm formation in Escherichia coli. Previously, we identified more than 10 transcription factors that participate in regulation of the csgD promoter. After genomic SELEX screening of regulation targets, an uncharacterized TetR-type transcription factor YbjK was found to be involved in regulation of the csgD promoter. In addition, a number of stress-response genes were found to be under the direct control of YbjK. Taken together, we propose to rename it to RcdA (regulator of csgD). One unique feature of RcdA is its mode of DNA binding. Gel shift, DNase-I footprinting, and atomic force microscopic (AFM) analyses indicated that RcdA is a DNA-binding protein with a high level of cooperativity, with which it covers the entire surface of probe DNA through protein-protein interaction and moreover it induces the formation of aggregates of DNA-RcdA complexes. |
| Links |
PubMed PMC3535384 Online version:10.1002/mbo3.42 |
| Keywords |
Biofilms/growth & development; Blotting, Northern; DNA Footprinting; DNA-Binding Proteins/genetics; Electrophoretic Mobility Shift Assay; Escherichia coli/genetics; Escherichia coli/physiology; Escherichia coli Proteins/genetics; Microscopy, Atomic Force; Promoter Regions, Genetic; SELEX Aptamer Technique; Trans-Activators/genetics; Transcription, Genetic/physiology |
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