PMID:23222720
| Citation |
Fried, L, Behr, S and Jung, K (2013) Identification of a target gene and activating stimulus for the YpdA/YpdB histidine kinase/response regulator system in Escherichia coli. J. Bacteriol. 195:807-15 |
|---|---|
| Abstract |
Escherichia coli contains 30 two-component systems (TCSs), each consisting of a histidine kinase and a response regulator. Whereas most TCSs are well characterized in this model organism, little is known about the YpdA/YpdB system. To identify YpdB-regulated genes, we compared the transcriptomes of E. coli cells overproducing either YpdB or a control protein. Expression levels of 15 genes differed by more than 1.9-fold between the two strains. A comprehensive evaluation of these genes identified yhjX as the sole target of YpdB. Electrophoretic mobility shift assays with purified YpdB confirmed its interaction with the yhjX promoter. Specifically, YpdB binds to two direct repeats of the motif GGCATTTCAT separated by an 11-bp spacer in the yhjX promoter. yhjX encodes a cytoplasmic membrane protein of unknown function that belongs to the major facilitator superfamily of transporters. Finally, we characterized the pattern of yhjX expression and identified extracellular pyruvate as a stimulus for the YpdA/YpdB system. It is suggested that YpdA/YpdB contributes to nutrient scavenging before entry into stationary phase. |
| Links |
PubMed PMC3562098 Online version:10.1128/JB.02051-12 |
| Keywords |
Binding Sites; DNA Footprinting; DNA, Bacterial/genetics; Deoxyribonuclease I; Escherichia coli/genetics; Escherichia coli/metabolism; Escherichia coli Proteins/genetics; Escherichia coli Proteins/metabolism; Gene Deletion; Gene Expression Regulation, Bacterial/physiology; Genotype; Membrane Transport Proteins/genetics; Membrane Transport Proteins/metabolism; Plasmids/genetics; Promoter Regions, Genetic/genetics; Protein Binding; Protein Kinases/genetics; Protein Kinases/metabolism; Pyruvates/metabolism; Transcription Factors/genetics; Transcription Factors/metabolism; beta-Galactosidase |
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