PMID:23207917
| Citation |
Peters, JM, Mooney, RA, Grass, JA, Jessen, ED, Tran, F and Landick, R (2012) Rho and NusG suppress pervasive antisense transcription in Escherichia coli. Genes Dev. 26:2621-33 |
|---|---|
| Abstract |
Despite the prevalence of antisense transcripts in bacterial transcriptomes, little is known about how their synthesis is controlled. We report that a major function of the Escherichia coli termination factor Rho and its cofactor, NusG, is suppression of ubiquitous antisense transcription genome-wide. Rho binds C-rich unstructured nascent RNA (high C/G ratio) prior to its ATP-dependent dissociation of transcription complexes. NusG is required for efficient termination at minority subsets (~20%) of both antisense and sense Rho-dependent terminators with lower C/G ratio sequences. In contrast, a widely studied nusA deletion proposed to compromise Rho-dependent termination had no effect on antisense or sense Rho-dependent terminators in vivo. Global colocalization of the histone-like nucleoid-structuring protein (H-NS) with Rho-dependent terminators and genetic interactions between hns and rho suggest that H-NS aids Rho in suppression of antisense transcription. The combined actions of Rho, NusG, and H-NS appear to be analogous to the Sen1-Nrd1-Nab3 and nucleosome systems that suppress antisense transcription in eukaryotes. |
| Links |
PubMed PMC3521622 Online version:10.1101/gad.196741.112 |
| Keywords |
Base Sequence; Escherichia coli/genetics; Escherichia coli/metabolism; Escherichia coli Proteins/genetics; Escherichia coli Proteins/metabolism; Gene Deletion; Gene Expression Regulation, Bacterial; Genome, Bacterial; Peptide Elongation Factors/genetics; Peptide Elongation Factors/metabolism; Protein Binding; Transcription Factors/genetics; Transcription Factors/metabolism; Transcription, Genetic |
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