PMID:231461
| Citation |
Boy, E, Borne, F and Patte, JC (1979) Isolation and identification of mutants constitutive for aspartokinase III synthesis in Escherichia coli K 12. Biochimie 61:1151-60 |
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| Abstract |
We devised a procedure in order to isolate, in Escherichia coli, constitutive mutants for aspartokinase III synthesis, the first enzyme of the lysine regulon. It consists of the introduction of a limiting step in lysine biosynthesis, by the use of the partial suppression of a nonsense mutation. For the first time we could isolate many constitutive mutants. Their characteristics (cotransduction with the lysC structural gene; no effect on the synthesis of other enzymes of the regulon; cis-dominance) lead to classify these mutations as operator-type. The fact that no repressor mutations could be isolated is discussed. |
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| Keywords |
Aspartate Kinase/genetics; Escherichia coli/enzymology; Escherichia coli/genetics; Escherichia coli/isolation & purification; Lysine/pharmacology; Mutation; Phosphotransferases/genetics; Species Specificity; Transduction, Genetic |
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