PMID:2270076

Citation	Ezaki, B, Mori, H, Ogura, T and Hiraga, S (1990) Possible involvement of the ugpA gene product in the stable maintenance of mini-F plasmid in Escherichia coli. Mol. Gen. Genet. 223:361-8
Abstract	The seg-3 mutant Escherichia coli does not support the maintenance of mini-F plasmid at 42 degrees C. We cloned the chromosomal DNA segment of the wild-type strain W3110 that complements the Seg- phenotype of this mutant. Cleavage mapping of this segment showed that it was derived from the 76-min region of the E. coli chromosome map. Complementation tests using plasmids carrying subcloned DNA segments suggested that the seg-3 mutant carried two mutations that additively affected the maintenance of mini-F plasmid; one was in the ugpA gene and the other was presumably in the rpoH gene. We generated a disrupted ugpA null mutant and found that the mini-F plasmid was unstable in this ugpA null mutant even at 30 degrees C. This suggests that the ugpA gene product is required for the stable maintenance of mini-F plasmid.
Links	PubMed
Keywords	Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Biological Transport; Cloning, Molecular; Escherichia coli/genetics; F Factor; Genes, Bacterial; Genetic Complementation Test; Heat-Shock Proteins/metabolism; Mutation; Phenotype; Phosphates/metabolism; Restriction Mapping
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