PMID:2266491
| Citation |
Jung, H, Jung, K and Kleber, HP (1990) L-carnitine uptake by Escherichia coli. J. Basic Microbiol. 30:507-14 |
|---|---|
| Abstract |
The uptake of L-carnitine by Escherichia coli 044 K74 which is able to metabolize L-carnitine to gamma-butyrobetaine under anaerobic conditions was studied. The uptake system of E. coli was induced in the presence of L-carnitine or crotonobetaine. The optimum influx of L-carnitine was found to take place at 37 degrees C in phosphate buffer, pH 7.5. On the basis of the temperature dependence of the uptake rate an activation energy of 53.5 kJ/mol was estimated. The transport of L-carnitine was energy dependent. The kinetics of L-carnitine entry followed the Michaelis-Menten relationship yielding a Km value of 5.3 x 10(-4) M and a Vmax of 154 nmol/min.mg protein. The transport system showed considerable structural specificity for trimethylamine carboxylic molecules. The results suggest that E. coli possesses an inducible, active and carrier-mediated uptake system for L-carnitine. |
| Links | |
| Keywords |
2,4-Dinitrophenol; Anaerobiosis; Betaine/analogs & derivatives; Betaine/metabolism; Binding, Competitive; Biological Transport, Active; Carbonyl Cyanide m-Chlorophenyl Hydrazone/pharmacology; Carnitine/metabolism; Dinitrophenols/pharmacology; Escherichia coli/drug effects; Escherichia coli/metabolism; Hydrogen-Ion Concentration; Oxidation-Reduction; Temperature; Uncoupling Agents/pharmacology |
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