PMID:22558308
| Citation |
Eswaramoorthy, S, Poulain, S, Hienerwadel, R, Bremond, N, Sylvester, MD, Zhang, YB, Berthomieu, C, Van Der Lelie, D and Matin, A (2012) Crystal structure of ChrR--a quinone reductase with the capacity to reduce chromate. PLoS ONE 7:e36017 |
|---|---|
| Abstract |
The Escherichia coli ChrR enzyme is an obligatory two-electron quinone reductase that has many applications, such as in chromate bioremediation. Its crystal structure, solved at 2.2 Å resolution, shows that it belongs to the flavodoxin superfamily in which flavin mononucleotide (FMN) is firmly anchored to the protein. ChrR crystallized as a tetramer, and size exclusion chromatography showed that this is the oligomeric form that catalyzes chromate reduction. Within the tetramer, the dimers interact by a pair of two hydrogen bond networks, each involving Tyr128 and Glu146 of one dimer and Arg125 and Tyr85 of the other; the latter extends to one of the redox FMN cofactors. Changes in each of these amino acids enhanced chromate reductase activity of the enzyme, showing that this network is centrally involved in chromate reduction. |
| Links |
PubMed PMC3338774 Online version:10.1371/journal.pone.0036017 |
| Keywords |
Chromates/metabolism; Chromatography, Gel; Crystallography, X-Ray; Escherichia coli/enzymology; Escherichia coli Proteins/chemistry; Escherichia coli Proteins/metabolism; Flavin Mononucleotide/metabolism; Hydrogen Bonding; Models, Molecular; Mutant Proteins/chemistry; Mutant Proteins/metabolism; NAD(P)H Dehydrogenase (Quinone)/chemistry; NAD(P)H Dehydrogenase (Quinone)/metabolism; Oxidation-Reduction; Protein Multimerization; Protein Structure, Quaternary |
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