PMID:2254324
| Citation |
Deguchi, Y, Yamato, I and Anraku, Y (1990) Nucleotide sequence of gltS, the Na+/glutamate symport carrier gene of Escherichia coli B. J. Biol. Chem. 265:21704-8 |
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| Abstract |
The nucleotide sequence of the gltS gene coding for an Na+/glutamate symport carrier of Escherichia coli B has been determined, and the amino acid sequence of the carrier protein was deduced. The predicted glutamate carrier consists of 401 amino acids with a molecular weight of 42,455. A Shine-Dalgarno sequence and putative promoter sequences were found in the 5'-flanking region of the putative gltS gene. The predicted protein is very hydrophobic (73% nonpolar amino acids), and judging from its hydropathy profile, the protein is composed of 12 hydrophobic membrane-spanning segments with a mean length of 21.6 residues/segment. A typical rho-independent transcription termination signal was found downstream of the gltS gene. We found a conserved alignment of 5 amino acid residues (Gly42--Ala82-X-X-X-X-Leu87-X-X-X-Gly91-Arg92 ), which commonly exists in four Na+ symport carrier proteins, the glutamate carrier, and the proline carrier of E. coli, and the Na+/glucose co-transporters of rabbit and human intestines. We propose that this consensus sequence (or motif) may play an important role in cation recognition or binding in the Na+/solute symport reaction. |
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| Keywords |
Amino Acid Sequence; Amino Acid Transport Systems, Acidic; Bacterial Proteins/genetics; Base Sequence; Carrier Proteins/genetics; Escherichia coli/genetics; Escherichia coli Proteins; Genes, Bacterial; Glutamates/metabolism; Membrane Proteins/genetics; Molecular Sequence Data; Restriction Mapping; Solubility; Symporters; Terminator Regions, Genetic |
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