PMID:22539721
| Citation |
Seth, D, Hausladen, A, Wang, YJ and Stamler, JS (2012) Endogenous protein S-Nitrosylation in E. coli: regulation by OxyR. Science 336:470-3 |
|---|---|
| Abstract |
Endogenous S-nitrosylation of proteins, a principal mechanism of cellular signaling in eukaryotes, has not been observed in microbes. We report that protein S-nitrosylation is an obligate concomitant of anaerobic respiration on nitrate in Escherichia coli. Endogenous S-nitrosylation during anaerobic respiration is controlled by the transcription factor OxyR, previously thought to operate only under aerobic conditions. Deletion of OxyR resulted in large increases in protein S-nitrosylation, and S-nitrosylation of OxyR induced transcription from a regulon that is distinct from the regulon induced by OxyR oxidation. Furthermore, products unique to the anaerobic regulon protected against S-nitrosothiols, and anaerobic growth of E. coli lacking OxyR was impaired on nitrate. Thus, OxyR serves as a master regulator of S-nitrosylation, and alternative posttranslational modifications of OxyR control distinct transcriptional responses. |
| Links |
PubMed Online version:10.1126/science.1215643 |
| Keywords |
Anaerobiosis; Culture Media; Escherichia coli K12/genetics; Escherichia coli K12/growth & development; Escherichia coli K12/metabolism; Escherichia coli Proteins/genetics; Escherichia coli Proteins/metabolism; Nitrates/metabolism; Nitric Oxide/metabolism; Nitrites/metabolism; Oxidation-Reduction; Promoter Regions, Genetic; Protein Processing, Post-Translational; Regulon; Repressor Proteins/genetics; Repressor Proteins/metabolism; S-Nitrosothiols/metabolism; Transcriptional Activation |
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